Microplastic-Induced Oxidative Stress on Intestinal Barrier Function inInflammatory Bowel Disease

In recent years, there has been an apparent rise in the incidence and prevalence of inflammatory bowel disease (IBD) across Asia, especially within urban areas and among younger age groups. Environmental elements like dietary changes and pollution exposure have been put forth as potential explanations for this trend. Microplastic contamination that exists in the environment, food and drinking water is particularly intriguing since it may have a big impact on how the disease develops. The precise mechanisms by which microplastic influences the severity and progression of IBD remain unknown. The study aims is to investigate the potential relationship between microplastic exposure and the IBD patient severity, as well as the underlying mechanisms by which microplastics may affect IBD. A total of 64 participants for each group that contains mild to severe IBD and healthy control will be recruited. Stool will be collected to identify the chemical composition of microplastic using fourier-transform infrared spectroscopy. Stool will be sent for Ilumina platform sequencing of the 16S rRNA V3 region. Micro/macronutrients intake will be calculated using a three-day record. A short questionnaire will be used to assess physical activity. Blood will be collected to assess for oxidative stress markers, superoxide dismutase, catalase, and glutathione peroxidase levels using ELISA. With the aid of barrier assay, the intestinal permeability of tissue biopsies taken from IBD patients who had microplastics contamination will be examined. Nanoparticles will be detected using ion coupled mass-spectrometry. The study could reveal new insights into the disease’s pathophysiology and potential factors to consider before making a therapy decision. It also lead to recommendation to reduce microplastic exposure in IBD patients based on this solid evidence. The findings may have important implications for public health, by informing policies and practices that attempt to reduce the negative impacts of microplastic exposure on human health